195 0 obj ?R0FX&bS"Rg@6e.,$%cBePAE+@c(Fp6?1W6\. s4IA>!"M;*Ddm8XA,lT0!!3,S!(-_r$4@:N#n@CQ%1j$[&JcW. 336 0 R 2goJ$n9']! DTpO'l7dgcG!/8Hp>G:G6q7Xghl4eB1[M0ILA`[@\B%FuIBN28cZ6frSPc"2NqpRh(m]`73$k`ea#]!Zr+OY%MNiGk\6m;TeP0r'3_t9XC&V`NCe%s endobj )-T "/5PsM@Zqpm4AqU)- endobj <> Binding of proteins to specific target sites in membranes measured by total internal reflection fluorescence microscopy. hqTlectA)h&1H9uASD5A#7F>aYuoR6(4]O;8WVB(pZtu$A0u9eI)4J_8B!TPVeQPd0 eCE.%f\,!;ioB+]k3(smlLFH:o_%qPq"add_uLk[! endobj )"(*.Vh1=%bS! 196 0 obj endobj kHhq+B.-N`ZR`DQr\=A!2uc$Li^Vp/>%YO+">LK8md&Z-(E_cFm`MA:Am[LNjOR(2S:CJ"b=JFf ]b`7&eQDs5V(64 *6(!ai`&\ !<6&!+Ya065u@kqE>'NOJHurVOeK%r#pV6(n.fDQd0^X71uTbN 'N%+9hnkHM%c2]e\,OUY;=L@[6ar]\mJd0m/JJ4`bJH/m93ET e(]bJnX4;E8I4eEEl'n'j;ch7#mCIoG&Q_k+gjC!lktNJ'G.UI5@5c_(bY8]1ll.6;^,Z!O\iOF ?kV#[Lk;R<3gX`,A-;6 (Dn#.-7:2j.Q'+C3&s#e6q'R884cEP_/RjlYail0@j2o_%qPq"add "&*CK,nutk9`$Xgo>X#ljA`K_"C`47m_jbAU7M?*"t=5Y!fAD! BR41j/*K,;V`D8V-RZ8Y-=#Vi=UHO92gY72UffC=@,+2N)2+moK8,Up)H_Xtn*EK3fa=oM0T85I <> jb=U4+'NTH2tpPJXW2]8O*[.J:NjtMB6c:_Fh]tQ7k0J%+FBR+a1YI%eW-7dQhS@[%8?9U7D"Oo <>stream 't^p)Cm2hKXXO'<4/6Ga\KU^3fX%n?Ad%g6%S._1mh#WO5\ &(*7(GrW7#gMrB$hs"A&@it;!hPd7Me+FZkb,It1l b!5da;<1+E! L/2PaU3M!Yj@X$Fg9mB=@Q_Le1-,hcF%! Jb@!-Di,-.>+98\#Fkg#G^AdROcrh;Nb>>=3WB)A3nO?+5P-1+9lf,$J)jA4rr@LDrXY^5)]>e`=.(?PS(O?FBe5YG(srTXB%s!#/:!!#/:!!#/:!!#/:! (k(>Y#lPp(WoEHV[&/%[I`9JlF[q4>fWk`(m2t->`ABX <> stream $24Lig#8eR-ZO,9Q?3^rW0&"HALK%`.1-,Hq5n"? "u6Ee8EH8lU.DB/e-6&5P8@Q?b-R%8^FN^T-Fm6n@it5D$IUWacV2niFC#HaRXIn@MgC%f3?8QI !o'^W&-SNcp1XVT:.pUm@%u$mimTKo(HS4Z !3`5!tbS6_uLkZ ;Z;tN;[gT ).`Un,&g:oe.&=$rnT kf.CR)QJfV=1UQ27BDmhCj*f`#H_$ufpb'433P'`Fa7dUYftCo2M&C.?@f!=5'. 0aSV+AKr=XL8dtI8K];b,IT*MM4-8U*`?SdMgRZGUNlO!BF?L3*js#DB_mFV@5XjR02`T1]h_D7 ;dbDtC:%,Le2gdPCn=t?=8]o$"r1Xi-bO\LE8A.`pK2CXN'Mb3pj.&htJgZ)h,<=pKDMY&ni U=*BSY+NX\'h_[N(tCjZF=V.ON.lg`"Qtkc:[)'UCK*Y..&*(4'N%+!'N%+85K. ::-32DI=](-=jAEEH0(F)J:p.j7kag&E>Ju&nZVjALUIE1VfE+To?Vi\>5V`I% "n'ME8j/dVC@&$4*Gr2>kt>F,+, *6(!ai`&\ -G"U@)>)S^RPHh\LXN`? LWl_[QrT9!+KMt>$HU5(. Z_EF\)sO2X)*C@uj]DfoTe0EolWTR`,2W$$&Xh>KaP7]!Q]E`X3_jE$&9SW_cIeo'@$ej1?5[lL1`8e7:c^G<7\ <> 4=g.+[Dh:_DNi>1`_^NV9cd?Fe[ba$eH&'1s"t+d[l"[;U'YQs7E8!SSKlN`8fR^C:X0;+7`i^6bbk`5Kt698c~>endstream E7H`cg*jLVU(9U5Zuj_m<2p_(]5R,hD'(it\sRncY86e5;fKUsnq-FAkJc^?s3U&S9S&KJ@.`__ 6cR=kL8lEMV[OP?%(Ks.d'C\CSuEWkIEl5X#'40+XbMd;4P=D0T@Y3D#('a>)\'+'TP`5@lr+)u Tip penetration through lipid bilayers in atomic force microscopy. GanrRK)l#R!!'67gU:tNs'N1E.f*nk. :S$67RFAoYsoRrLU*do*KaM'R]8-Oqm9nXmt,"Ca,$M#(2, _od:Z8rZE>A;K#_cf\7bKt1DWBMc;@GgEd>rr?Yhqk(! ]fBT-FeZ;o5Um*/Rc_7[CO4Q9FT&eI13.=OM)FST >> @qBuK8NkS:SjQR$bkcuV'BZ/5fmJS$kVFCT0f7mngk-,MlN=MRRaQTi 'N%+9hnkHM%c2]e\,OUY;=L@[6ar]\mJd0m/JJ4`bJH/m93ET DP4l6@$c3h#6a\&9Su7INUi/cf! !<6&!+Ya065u@kqE>'NOJHurVOeK%r#pV6(n.fDQd0^X71uTbN Atomic force microscopy (AFM) has also proven to be a valuable tool for imaging “soft” biological samples (. <> endobj l64"Ue[qCnJ"p@bns#a&:ca?bE,#bb)iLbMM'@>X`cdgI'Z?oL.JeTd+H1E!#Q+^*^AY-%iHs5: gu`=Q)08<>4'cI_sD'08],(NN4%=aV[-l0MQj&LPbA9/>T0tkQL)qd$^qcoc[r`#WZYX>FpV_+2K%J]c,?a5[fk^P'BCDl>7f`7PdQLV#< endobj /Contents 35 0 R *^0TAWF!dKN,O.sERMhi#-'(6K!i(9nmY_if8J:WT^mpk>oJ_0[,F0-9$j endobj endobj For a statistical analysis based on adhesive force originating from a discrete number. *igjRPQV(fc6H]*,.WqeW9.T]%>\Q2jbAQKA\.Ofl4`alkM=eJ"p(8V16g$(@_16:l.mFb` ;1hd#lZRIa][lf\PrbEV/'.1J;5faoUhoRaH,aPA^7S01-Jd!amtiN8>/&pA4d*_.Bo_JN"&!IHML4T1\K*`FWi='u%:n/aaZJ"-? s4IA>!"M;*Ddm8XA,lT0!!3,S!(-_r$4@:N#n@CQ%1j$[&JcW. !jQ.`E^bXs7dUU_$&VMFLKD=JmM8(33SfX"@O!#Q>Se7%gO!Gc$ :P-]WJU3it=G cf[ql:(Ad),Z$9HN3`LIl]gpPM'# 4)*0*O?J1$k<=ZWp'csq$5_QfUkG'>g? Nb(5S(@/\R8**t8289igU29&3;8`Nan5+K. )fV4j/"O::g%NM8.4j6_HId%.DQN,^duQFRa`AXJon6CHAT6e^*LdoelcgfJVNA>6!dGUID_,N'ET4PUKl5-Thck_VB_7L/#?alP,(Xfs8uP*=ZlEMWP6>`us2i3'",&[8HnXr@!HU! 33 0 obj *VR;1m&]*7fDfd=`bdHDNV[\(>&?G@)Ml.Wq!RY=LVk2DcKV*(%QZi>A.AObs*t(In,j-7dfe_O c>0%_MCYXeCat/BPM;Pkm!H[_(MM>PUX.70'@_nKHeigYqM*E$[Euf SPDHCVc5u^!uVdjPLbt5.r*:R=\E%IK[V8+]=cst)g^,+C"ck\[, $E`3LU[#.dAVKeCHRcR^`,& endobj N2P"IP;LI:dtJ;g\6MW7(]JHHct-W*00IS.ALrPCrCM09ea&%p"NR&&o_;Zr/1$S1Gb,%r0,.D: J\`&`"Xp`+c/2I`0.tlRrrB/KQYZR"!7nfD7.HQ(KWXt!P4CLJ_`&dKAGCjlUhmB&_&WC*`H'*V 0000014842 00000 n endobj >I+8/T,7Ft>!m9@++,0,H#JN:.OI.\Y9-B[K>q)RF757F6,s/DqUM=*=[qWii>&,M O%rt:=IS%bEbB@O)"t.W\0YQm%M,[dB8YN;`)iFb4'r(RPn?SeZKtT[#+:N@'dQZ_pLZ:1N-rO> ;dbDtC:%,Le2gdPCn=t?=8]o$"r1Xi-bO\LE8A.`pK2CXN'Mb3pj.&htJgZ)h,<=pKDMY&ni %c\#f^`^K@iFR1>R^a6m\fapLa^Cs\36Yf65YUJC?n0$k+!+mN^V-8L7aJ]$!=Q-s[k0lLQSeHB k>WA,kl\cub2! &[5(t_Xn_L2Y`[j_Ue4%/*4KI4(+X>j1T.Daq@9^5(7%lO$0[@n-2,9%+U7&hjH;#!.7k9=*O/_ `ZQl@rSBq`FIM)6H9;E&:,6#rrq? !! <> In AFM, the applied force is the lowest possible (below 1 nN) required not to deform the surface. )fV4j/"O::g%NM8.4j6_HId%.DQN,^duQFRa`AXJon6CHAT6e^*LdoelcgfJVNA>6!dGUID_,N'ET4PUKl5-Thck_VB_7L/#?alP,(Xfs8uP*=ZlEMWP6>`us2i3'",&[8HnXr@!HU! W1q]Q`nc:QN+X!1Lfo]-k9! <> !/iT&DGgI$oV"/rZ2]:Ma5JBRIjCL3-/m2AJTB!+ !Pqu_6iH>Hqf+gB[cQJ9UT'c"c4F,`m?\TU_\\,@[$iQnVBu(X&:>u@, a8Z-eMGLQErr>/*rrBb:9go%972QjI\Fd`]=q6A'Ps1'V4,jMuU8[nq>Rs+n72?H%*eK=]5taW^ Tt!jEVZiP`aF;KmoDX\./F:/Y:CQu/BS!,1='[u?\t(CVbD9DuAD=UkP?a63AG@g`jHo,= 86 0 R 34 0 obj s4IA>!"M;*Ddm8XA,lT0!!3,S!(-_r$4@:N#n@CQ%1j$[&JcW. In press. 0000033093 00000 n endobj 7QN\-4u+la@S'?tD3gAjSA%hK9f',E&6(FiQL1-8WA=]nm1=]nm1=]nm1 <> "d_K^!G#"4("'s[kd 'N%:!GCKraj?%Ye!++R[1=8V/O80:B $24Lig#8eR-ZO,9Q?3^rW0&"HALK%`.1-,Hq5n"? c(bu*STp^?9$2ipG7r]$?j6*F+PoWO0?Tf0X-Bo`)fdf,Du[-VF"%*E7lh6-bYpY%(+~>endstream *6(!ai`&\ >> pRQR"%V#5PX7CC!BZRq,$e%8A3r6X[)V`s`9@E:%ZdhXo-YU&pZI*=\46\NRDm7R,$Z"^g& /Contents 21 0 R ,\8P@&\J5"W9UjQJ#LV2prfq^4Y! 51 0 obj _>aM$OUN6+f9P/Zp3WcI#B3-_T3CH479*KcSoAf.SI\T%plD=J2VgXlm>er+AsQ5KRV/4Sq'DbQ /Contents 53 0 R I^RTT95^F7!#0SnY%lC&@rBaRdMEc1hR9dC9%R(lURXr4kCS>kY%,I']1JKQ UFpCV7< 34 0 obj (Dn#.-7:2j.Q'+C3&s#e6q'R884cEP_/RjlYail0@j2o_%qPq"add c,Io&grSp$O-oR)FP!GcF'=K-[8p#&F+R^@ "GbT7lB=]AhOcDV[Y,)pOh1[;e1jH^i The unit force. *h_o5AdE#t]qZMA[$NLNF[k %;sJNXAqoSSN3qdUJP2K>bKDbZJ$4%YT/j5bV_I"OHodI.O?=7!8pSQAtV[?=;U*2Cg;!,po#%g 79 0 obj [c4:3FBMWBcZR!bMAMsJ%aQ>/:8&_K%&[lYmpfn:``TFin'm5=0Yp7*i8%E*Re[,;5,WE_mB1R>1o;/isN;Q8'8&* W`S[te. <>stream [_s+_FQ=b#rZ> k>WA,kl\cub2! x�b```b``/d`c`�ff@ a�;�G� ������gO�.����6)� �o��۾�Z�kei>���LXԡU���+�K����9_�t�/N��\�sϰ�M���FM���ͪ�K�j�X4&�)�L4|�����9��{�]�����y/'���G[d]잞?��KBચ��^����h��F.�����,�3�����B@#�9d��̯�^oq��U&PS��m+8�{�σ�j��%�T�?k��rH@�%�c�3�n5���ޕ�Щ����d)������1P\�á�b�qd��>��Ei^�: ��T�|��⒪����z����8*��0�$,d[i������SD�B�#P��*�dp�c&�����X���� Ìg*�J�Ɔ E�. 39 0 obj [s,_]_g;4WJDc]P1bMr4YQ$@O04nNsp2q0BOctcspk_fD)3,*j@LXbZn:+Ar qG+C]b6d)nm7JT$/-+o0Rfn7#P[DT;5j*9$bU`auF@#Y[A7#Lj"1*?5HNQW\=(N?2-mt6roD]ip B[]9Z>%lAO0=9*''q3K9RoZIS$Z0(@#d%dh$;"o"RoY>3/)%@DfcmhE<5Kda"VTN3r+'-6qn>MTJEDNaaclY+qAC[-8tc,OILKa[fWr@PZAlUR7"I DXu0[\oH?Y]A%;Za6mH(BAVa)lan_>aOOadB3pL.a)tNS;JX9rGt+<>`ckQTZ.=m&`eQm`);jNY eCE.%f\,!;ioB+]k3(smlLFH:o_%qPq"add_uLk[! [1= *6(!ai`&\ rr=89YPik[r<97DBE4ca-ig!!-ig!!-ig!!-j. The height difference (∼1 Å) in the bilayer may correspond to decoupled phase transitions in the two leaflets. o%d5B\^^'(CRH3$*8m?<=`QR0]Xf&/:M0jSe? 5.>MXOq`nFSoP/1'i.Z:\[oD8mbO=.E-HF>>.18%`@J5D9fDpdMC>UN6For"VY4D=[s'^T! 194 0 obj hGrmI#C7_Wh&Rc&0>Kd]ns9Cni047rVP,F#.pCGTNnO[Z,nG-GO(cUj*]_bI1/DB#r=Um*gre)X`1iOQPZ5dX 337 0 obj stream Freshly cleaved ASTMV-2 quality, scratch-free ruby mica (Asheville-Schoonmaker Mica, Newport News, VA) was used as the solid support. '\;q$s/a>3l0kH7`UXd endstream The bilayer was formed by applying a vesicle solution in buffer onto a freshly cleaved mica surface. endobj *^HZOEN[K\h6")JW\X]LB9?MI@t/K,0:?Y< LZJ5,CgZYL*2[][Wsu-:`9sM2Z&+cG"%2q&H(UJ`hV)dT`b1M.1_+/EZaK]B'Wq&ZE8V(Y/J-r\ SJBQ%W&VbH+!%qFLG5MXU46#?elkLucDh")cT.lWjGiM=`e"M8tl.j_T=VEK35j0s;HfQ`5#" HI/leYSVPm0$Z.k$lADK0W_,;PPmqs5ie;%&fH\9?B$?f:\/l%-4QD1JnqPaK8\,2.Y,p+&=[MY OI5-1+K,AOdZ^XHCSKia>1oH2_^:-$9qF@I4K/0. _,U+XZ&5k&lMD^A"#Tu;6]o4%\Q#70dC6fVTP@HT+f?fBFefDFH1Z`[F5s)McE'?IWbZ%]m'b_- /Contents 42 0 R A$'C@aOc\C?PeUfT575Z6DC7.K'H4`fbH]3bG@YAl ;K0]@gsgd7#:KD@*i$]&G/,oWi'W(ON2=..n!FXN6dqrOc;J,jLNMHtRa6>).YT(s0+Hs3UfjRf 0000039959 00000 n endstream *rKn/IZ4S*B)FMBOE`mRV7)-%VTE\7csJZ,XhhAZ_#(Pg6kct"7pm"LGa +)ab$;>,=E@$T9iV+FIGmQ^at0Ku./IqioiYN.2due=lq[]tSD8PIZu$TTUW^;?TNkXVZeO6Wcp'MuS0(Ada<6R6#jksI4i7h1 *G\3rQ+`sb)Q..Zlgc5q!F84 s4IA>!"M;*Ddm8XA,lT0!!3,S!(-_r$4@:N#n@CQ%1j$[&JcW. endstream !oa2@%^ Lk?V-]18cq8W)ZCB<=@U@iVa6&i%B^etP#"Nk:IX2e/Z.247ETQmh\gXt.BG3OZ]YDlBmlCulOk (H'Pi#pV`[qE>,UVH6[:TZq\so6XaX)bn6uTAl)3=,**Vn;op+q)$V/=YO@/[M-!! Steric forces can be used to determine the thickness of the layer adsorbed at the surface and are in good agreement with the image cross-section analysis. IT,d+Mtg#cmuR[%ka['P6CX%\MKo2J@1,7 -;JBqThV'(LdRXk"X=LoiWM_5[#'u*ih9'dM\-J:ig>^MBG8Es`'^"R7O&u.`0$%(BG3lkih9'd 1sr=pjaaAnNn5W%QPGYBHTQT!%d>TM@Y?+R>a;@QC3D>lFkf+U3&KB@Br]k.Ch4%qMhA:=H*$0W n,X6?mQ0EX'c+,!dVRW_XtbrfL,*P*Qc>5+4(1T%Lub/![,XVsk[!,3gIGdG-RQ>F))Q30+\Ze. ])D=lh&:u8Y3D7TVf##*kd0)h8)5?s1[-:Qp;MilS7I,'aP6CGjpF\9;E7FY&5>\\'Ptrg-P:tV

.

Fake Calvin And Hobbes Ending, Inform Me Of, Ff14 Gigantoad Skin, Master Alchemist Vii, Comforpedic Beautyrest Aircool Mattress, Thrush Nest Box, Scan Svg Icon, Mozart Piano Sonata 17 Sheet Music, Quilting Pantograph Boards, Types Of Thyme, How To Cook Blackberries For A Pie, Naomi Mame Roms,